Thus, the plasmid sequenced in this study will expand our understanding of GES-type β-lactamases and provide insights into the genetic platforms used for the dissemination of GES-type genes.Ĭitation: Dang B, Mao D, Luo Y (2016) Complete Nucleotide Sequence of IncP-1β Plasmid pDTC28 Reveals a Non-Functional Variant of the bla GES-Type Gene. The enzyme produced by the bla GES-5-like gene cassette may be a new variant of GES-type enzymes. The mutations resulted in 7 amino acid substitutions in GES-5 carbapenemase, causing the loss of function of the bla GES-5-like gene on plasmid pDTC28 against carbapenems and even β-lactams. A bla GES-5-like gene was found on the accessory region, and this bla GES-5-like gene contained 18 silent mutations and 7 missense mutations compared with the bla GES-5 gene. The backbones of plasmid pDTC28 and other IncP-1β plasmids are very classical and conserved, whereas the accessory regions of these plasmids are diverse.
Plasmid pDTC28 belongs to the IncP-1β group by phylogenetic analysis. The complete sequence of plasmid pDTC28 was 61,503 bp in length with an average G+C content of 64.09%. This plasmid confers reduced susceptibility to tetracycline and sulfamethoxazole. coli CV601 ( gfp-tagged) as recipient and indigenous bacteria from the sediment as donors. Plasmid pDTC28 was isolated from the sediments of Haihe River using E.
